技術支持Article
英文名稱：Carboxypeptidase B from porcine pancreas；Peptidyl-L-lysine(L-arginine) hydrolase； Protaminase
其他名稱：精蛋白酶；肽酰-L-賴氨酸水解酶；肽酰-L-精氨酸水解酶
CAS號：9025-24-5
級別：BR
分子量：34500daltons
活力：≥70units/mg
活力定義：One unit hydrolyzes one micromole os hippuryl-L-arginine per minute at 25℃,pH7.65
*PH：7.9
等電點PI：6.0
Chymotrypsin and trypsin：≤0.02%
產品描述：Catalyzes hydrolysis of the basic amino acids lysine,arginine and ornithine from the C-terminal position in polypeptides.A solution in 100mM sodium chloride.PMSF treated to inhibit tryptic and chymotryptic activities.
性狀：粉末。以酶原形式存在于生物體內
用途：生化研究。可專一性地從肽鏈的C端開始逐個降解，釋放出游離氨基酸的一類肽鏈外切酶。羧肽酶B只水解以堿性氨基酸（如精氨酸和賴氨酸）為C末端殘基的肽鍵。
保存：-20℃
Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and ornithine from the C-terminal end of polypeptides. The molecular weight is 34,500 daltons, the pH optimum is 7.9, and the pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine, lysine and ornithine. The enzyme is not inhibited by di-isopropylfluorophosphate (DFP), but it is inhibited by metal chelating agents, e.g., 1,10-phenanthroline.
Unit Definition: One Unit hydrolyzes one micromole of hippuryl-L-arginine per minute at 25°C, pH 7.65.
Related Products:
Carboxypeptidase Y (COY)
Chymotrypsin, TLCK-treated (CDTLCK)
Clostripain, Endo-Arg-C (CP)
Protease, Stap, Endo-Glu-C (STAP)
Trypsin, SeqENZ (TRSEQII) & Modified (TRSEQZ)Carboxypeptidase B, PMSF Treated
A solution in 100mM sodium chloride. PMSF treated to inhibit tryptic and chymotryptic activities. Chymotrypsin and trypsin are less than 0.02%.
Store at -20℃
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